16.  You harvest a skin graft and want to save the leftover in the refrigerator.  How do you do this?  How long is it good?  How is allograft prepared?

Answer:
In 1998, a study was done which compared storing human skin at 4 degrees in either saline, McCoy’s 5A medium, oxygenated McCoy’s and CO2 McCoy’s.  Skin stored in saline lasted one week, in CO2 supplemented it lasted less than one week, but in regular McCoy’s or oxygenated McCoy’s, it was viable for 4 weeks. So oxygenation does not improve the viable storage time and CO2 appears to be detrimental. In this study, a STSG was stored for 5 weeks in regular McCoy’s and still resulted in good take.
 

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